The operation of the pipette is the first step in scientific testing

Update:03-09-2019
Summary:

Many people who use pipettes for the first time always […]

Many people who use pipettes for the first time always think that the operation of the pipette is very simple, but it is not. The operation of the pipette is the first step in scientific testing and is a very basic test operation, but most people are biased towards the operation of the pipette. In fact, the operation of pipettes is a lot of learning. If you ask yourself a few questions, you can see if there is any deviation in your operation.

1. Have you ever experienced liquid suction into the piston during operation?

2. Do you pay attention to ensure that there is no liquid on the outer wall of the nozzle when you pipette?

3. After using the pipette, have you adjusted the pipette range to the maximum value?

4. After using the pipette, do you put the pipette on the table?

5. Is the pipette operated in the same way when moving a thick liquid and a general liquid?

6. How many pipetting methods does the pipette have?

Set capacity: When setting the required capacity, it should be adjusted to 1/4 turn larger than the set capacity value, and then adjusted to the set value. This can eliminate the mechanical clearance and make the set value accurate, that is, adjust the capacity first. The knob rotates more than 1/4 turn of the target capacity value and then down to the set value.

Aspiration operation:

1. Connect the appropriate nozzles;

2. Press the control button to the first gear;

3. The pipette nozzle vertically into the liquid surface 1-6mm (depending on the size of the pipette);

Note: In order to make the measurement accurate, the nozzle can be pre-washed 3 times, that is, the liquid is repeatedly drained 3 times.

4. Slowly slide the control button back to its original position.

5. Wait for 1-3 seconds for the pipette to move out of the liquid;

6. Slowly remove the nozzle to ensure that there is no liquid on the outer wall of the nozzle.

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