1. Use the right tip:
To ensure better accuracy and accuracy, it is recommended that the amount of pipetting be within the 35%-100% range of the tip.
2. Set the pipetting volume
Adjusting from a large range to a small range is a normal adjustment method, and rotating the scale counterclockwise
When adjusting from small range to large range, it should be adjusted to exceed the set volume scale and then adjusted back to the set volume, which can ensure the accuracy of the pipette.
3. Tip installation:
For most brands of pipettes, especially multi-channel pipettes, it is not easy to install the tip: in order to achieve a good seal, insert the pipette vertically into the tip, rotate it a half turn left and right, and tighten it. Some people will use the pipette to repeatedly hit the tip to tighten, but this operation will cause the tip to deform and affect the accuracy, and serious will damage the pipette, so this operation should be avoided. Some multi-channel pipettes are equipped with O-rings, and with the tip of the front stop point, the ideal seal can be achieved with a gentle press.
4. Tip immersion angle and depth:
The tip immersion angle is controlled within 20 degrees of inclination, and it is better to keep it vertical.
5. Tip rinse:
For normal temperature samples, the tip rinse helps to improve accuracy; however, for high temperature or low temperature samples, the tip rinse reduces the accuracy of the operation, so please pay special attention.
6. Aspirate speed:
The pipetting operation should maintain a smooth, suitable pipetting speed; too fast aspiration rate can easily cause the sample to enter the sleeve, causing damage to the piston and seal and cross-contamination of the sample.