To properly freeze Cryotubes, you should follow a few basic rules. First, you should not fill the tubes to their maximum filling capacity. This is because cells need a certain amount of room to expand during the freezing process. It is recommended to fill the tubes about 0.2 ml below their maximum graduation.
Freezing cryotubes allows researchers to preserve samples at extremely low temperatures. Cryovials, also known as 1 mL cryotubes, are used to store samples at temperatures as low as -196 degrees Celsius. These tubes meet specific pressure requirements for transport via airplane. To make storage easy, some companies manufacture tubes with a notch at the bottom that makes them easier to handle.
The resulting sperm and egg mixture is diluted 1:1 with 10% DMSO and 5% bovine serum albumin. The cryotubes are then placed on a floating rack and immediately placed in liquid nitrogen. Once frozen, the samples can be thawed in a water bath for six minutes and then at 15 degC for four minutes.
The Freezing Cryotubes EF6100 and EF600-103 are two types of cryotubes, used for freezing and thawing samples. Both have the same features, but have different cooling profiles. The EF600-103 is programmed to cool samples at a linear rate of -1 degC/min. Once the sample has reached the pre-programmed temperature, the sample is held at -80 degC for 1 hour, then transferred to the -80 degC freezer.
Controlled-rate freezers are the perfect choice for storing cryotubes or other precision samples. They offer a wide temperature range, with an end temperature of -180 oC, which ensures sample integrity. They can be operated manually or through a PC software program.
A controllable rate freezer contains three major assembly parts: the cold end 101 of the Stirling engine, a position for the temperature sensor, and a fixed sample plate. The sample plate is held in place by a set of magnets underneath it. The sample plate can be used for several experiments at a time, and the sample plate can also accommodate microtitre plates.
To freeze cryotubes, you need to prepare them first. Prepare the cryopreservation medium by adding 10% DMSO, 30% FBS, and 60% medium. Then, freeze the cells in this solution for several months at -20degC. Be sure to use a Mr Frosty freezer, as any other type of container will reduce their viability and slow their freezing rate. Once the cells are frozen, label them with their hPSC line, cell type, and passage number.
Then, freeze the tubes with a volume of 50 mL. You should have at least 1 x 104 cells in each tube. This volume will allow you to freeze samples without any liquid nitrogen.
Freezing Microtubes can be an efficient way to preserve biological samples. The containers have numbered grids that allow for sample identification and a rotor that minimizes condensation. These containers are available in five and nine-well sizes, and they can be used repeatedly to freeze and thaw biological samples.
Depending on the type of liquid, some tubes freeze and expand rapidly and others expand slowly. A slow freezing is recommended to preserve the sample and tube materials. The temperature of the liquid and the stress on the tube material affect freezing dynamics. The proper handling of the tubes can minimize the risk of breaking them.
PREV：The Study of Upstream Configurations for Freezing Tubes NEXT：Centrifuge Tube - What You Need to Know